Anti-Collagen VII (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-1558R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideCollagen VII
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SpecificityThis antibody reacts specifically with Collagen VII
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human Collagen VII
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Gene ID1294
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Swiss ProtQ02388
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationStratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsEBD1; EBR1; EBDCT; Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen; COL7A1
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Also known asCollagen VII Polyclonal Antibody
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Other nameAnti-Collagen VII Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GeneCollagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolMARCHF7, IGLVVII-41-1, SYT7
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Short nameAnti-Collagen VII
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameCollagen VII Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namemembrane associated ring-CH-type finger 7
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Synonyms gene
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Synonyms gene name
- axotrophin
- membrane-associated ring finger (C3HC4) 7
- membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ring finger proteins
- Membrane associated ring-CH-type fingers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (VII)-41-1 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (VII)-41-1
- immunoglobulin lambda variable (VII)-41-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-15
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynaptotagmin 7
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Synonyms gene
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Synonyms gene name
- prostate cancer associated protein 7
- synaptotagmin VII
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Synaptotagmins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data