CDC6 Antibody ( DCS180)

  • Catalog number
    MA1019
  • Price
    Please ask
  • Size
    0,1 mg
  • Target antigen
    CDC6
  • Clonality
    Monoclonal antibody
  • Clone
    DCS-180
  • Raised in
    mice
  • Type of the antibody
    IgG1 monoclonal antibody
  • Product form
    freeze-dried
  • Reacts with species
    Human
  • Analyses
    WB
  • Immunogen
    Recombinant human Cdc6.
  • Product configuration
    Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
  • Purification
    Ascites
  • Solubilization
    The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
  • Storage condtions
    Keep the CDC6 Antibody ( DCS180)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
  • Tips
    The CDC6 Antibody ( DCS180) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
  • Background
    In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
  • Related articles
    1. Gonzalez, S.; Klatt, P.; Delgado, S.; Conde, E.; Lopez-Rios, F.; Sanchez-Cespedes, M.; Mendez, J.; Antequera, F.; Serrano, M. : Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Yan, Z.; DeGregori, J.; Shohet, R.; Leone, G.; Stillman, B.; Nevins, J. R.; Williams, R. S. : Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Nat. Acad. Sci. 95: 3603-3608, 1998.
  • Gene Name
    CDC6
  • Protein Name
    Cell division control protein 6 homolog
  • Gene Full Name
    cell division cycle 6 homolog(S. cerevisiae)
  • Synonyms
    CDC18L|HsCdc18|p62(cdc6)|CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE|CDC6(cell division cycle 6, S. cerevisiae) homolog|CELL CYCLE CONTROLLER CDC6|CDC6-related protein|Cdc18-related protein
  • Uniprot ID
    Human(Q99741), Mouse(O89033)
  • Entrez GeneID
    Human(990), Mouse(23834), Rat(360621)
  • Properties
    If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    CDC6   DCS180  
  • Gene symbol
    CDC6
  • Short name
    CDC6 Antibody ( DCS180)
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative name
    CDC6 (antibody to-) ( DCS180)
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    cell division cycle 6
  • Synonyms gene
  • Synonyms gene name
    • CDC6 (cell division cycle 6, S. cerevisiae) homolog
    • CDC6 cell division cycle 6 homolog (S. cerevisiae)
    • cell division cycle 6 homolog (S. cerevisiae)
  • GenBank acession
  • Locus
  • Discovery year
    1999-08-06
  • Entrez gene record
    990
  • Pubmed identfication
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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