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Target antigen
CDC6
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Clonality
Monoclonal antibody
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Clone
DCS-180
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human
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Analyses
WB
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Immunogen
Recombinant human Cdc6.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the CDC6 Antibody ( DCS180)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The CDC6 Antibody ( DCS180) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
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Related articles
1. Gonzalez, S.; Klatt, P.; Delgado, S.; Conde, E.; Lopez-Rios, F.; Sanchez-Cespedes, M.; Mendez, J.; Antequera, F.; Serrano, M. : Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Yan, Z.; DeGregori, J.; Shohet, R.; Leone, G.; Stillman, B.; Nevins, J. R.; Williams, R. S. : Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Nat. Acad. Sci. 95: 3603-3608, 1998.
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Gene Name
CDC6
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Protein Name
Cell division control protein 6 homolog
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Gene Full Name
cell division cycle 6 homolog(S. cerevisiae)
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Synonyms
CDC18L|HsCdc18|p62(cdc6)|CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE|CDC6(cell division cycle 6, S. cerevisiae) homolog|CELL CYCLE CONTROLLER CDC6|CDC6-related protein|Cdc18-related protein
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Uniprot ID
Human(Q99741), Mouse(O89033)
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Entrez GeneID
Human(990), Mouse(23834), Rat(360621)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps