CD8 FITC Monoclonal Antibody (Clone OKT-8)

  • Catalog number
    6954-25
  • Price
    Please ask
  • Size
    25 tests
  • Antibody Target
    CD8
  • Target Alternative Name
    CD8
  • Antibody Type
    Monoclonal
  • Clone
    OKT-8
  • Species Reactivities
    Human
  • Immunogen Sequence
    Human CD8
  • Accession
    P01732
  • Gene Id
    925
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
  • Purification
    Affinity chromatography.
  • Application
    Flow cytometry
  • Positive Control
    FACS: Human PMBCs
  • Application And Usages
    Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.125 µg).
  • Description
    CD3 (T3), a complex T cell marker, is known to associate noncovalently with the a/b or g/z heterodimer of the T cell antigen receptor (TCR) to form the most complex transmembrane (TM) receptor structures. CD3 is specially engaged in antigen recognition and is known to play an important role in mediating signals that are critical for T cell development in the thymus, proliferation, and induction of T cell-mediated immune responses against infectious agents and also in the differentiation of T cells into effector and memory populations. CD3 usually expresses in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, but cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. Apart from its role as an important marker in the classification of malignant lymphomas and lymphoid leukemia, CD3 can also be useful for the identification of T cells in celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein. CD3 indirectly plays an important role in immunomodulation whereas the anti-CD3 antibody may be used in in vitro Treg assays to generate effector T cells. The CD3 complex contains γ, δ, and ε chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. The OKT3 monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. CD3 enhances the antigen recognition by signal transduction. The OKT3 antibody is an immunosuppressive, which has proven to be an effective therapeutic agent in liver, heart, and renal allograft rejection.
  • Storage Condition
    4°C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
  • Additional description
    Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
  • Conjugation
    Anti-FITC Antibody
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
    CD8   Monoclonal   OKT-8  
  • Gene symbol
    CD8A, SNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
  • Short name
    CD8 FITC Monoclonal Antibody (Clone OKT-8)
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
  • Isotype
    Mouse IgG2a, Kappa
  • Label
    FITC
  • Alternative name
    CD8 fluorecein monoclonal (antibody to-) (clonality OKT-8)
  • Alternative technique
    antibodies, fluorescine
  • Clone name
    OKT-8
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 2/OR2-8 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 2/OR2-8
    • immunoglobulin kappa variable 2/OR2-8 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa (IGK) orphons
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 35
  • Locus
    8
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
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