Anti-UBE3A/E6-AP (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-11776R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideUBE3A/E6-AP
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SpecificityThis antibody reacts specifically with UBE3A/E6-AP
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human UBE3A/E6-AP
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Gene ID7337
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationE6-associating protein is a component of the ubiquitin-mediated proteolytic pathway, which selectively targets proteins for degradation by the 26S proteasome. Ubiquitin (Ub) is directly conjugated to protein substrates by the transfer of Ub from an E2 ubiquitin conjugating enzyme to the target protein. This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such as E6-AP. Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, HHR23A. Previous studies have indicated that E6-AP associates with the human papillomavirus E6 oncogene, which complexes with p53 and thereby potentiates E6-AP mediated ubiquitination of p53. Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, and, in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which is characterized by severe motor dysfunction and mental retardation.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsANCR; Angelman syndrome; AS; CTCL tumor antigen se37 2; E6 AP; E6AP antibody E6AP ubiquitin protein ligase; E6AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; EPVE6AP; HPVE6A; Human papilloma virus E6 associated protein Angelman syndrome; Human papilloma virus E6 associated protein; Human papillomavirus E6-associated protein; NY REN 54 antigen; NY REN 54 antigen; Oncogenic protein associated protein E6 AP; Oncogenic protein associated protein E6AP; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY REN 54; Renal carcinoma antigen NY-REN-54; UBE 3A; Ube3a; UBE3A protein; UBE3A_HUMAN; Ubiquitin protein ligase E3A; Ubiquitin-protein ligase E3A.
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Also known asUBE3A/E6-AP Polyclonal Antibody
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Other nameAnti-UBE3A/E6-AP Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolUBE3A, SNHG14, JUND, TFAP2A, TFAP2E, PTPRCAP, AP5B1, TFAP2B, KLF12, TRRAP
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Short nameAnti-UBE3A/E6-AP
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelAP
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Alternative nameUBE3A/E6-AP Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Alternative to gene targetubiquitin protein ligase E3A, ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, ligase activity, nuclei, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247,533136
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Gene info
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Identity
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Gene
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Long gene nameubiquitin protein ligase E3A
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Synonyms gene
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Synonyms gene name
- human papilloma virus E6-associated protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-10-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HECT domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA host gene 14
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Synonyms gene
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Synonyms gene name
- UBE3A antisense RNA 1 (non-protein coding)
- small nucleolar RNA host gene 14 (non-protein coding)
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Synonyms
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Synonyms name
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Locus
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Discovery year2009-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNA non-coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameJunD proto-oncogene, AP-1 transcription factor subunit
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic leucine zipper proteins
- Jun transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametranscription factor AP-2 alpha
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Synonyms gene
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Synonyms gene name
- transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
- transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transcription factor AP-2 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametranscription factor AP-2 epsilon
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Synonyms gene name
- transcription factor AP-2 epsilon (activating enhancer binding protein 2 epsilon)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-11-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transcription factor AP-2 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadaptor related protein complex 5 subunit beta 1
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Synonyms gene name
- adaptor-related protein complex 5, beta 1 subunit
- adaptor related protein complex 5 beta 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2012-02-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adaptor related protein complex 5
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametranscription factor AP-2 beta
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Synonyms gene name
- transcription factor AP-2 beta (activating enhancer-binding protein 2 beta)
- transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-12-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transcription factor AP-2 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameKruppel like factor 12
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-05-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
- Kruppel like factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametransformation/transcription domain associated protein
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Synonyms gene name
- transformation/transcription domain-associated protein
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- SAGA complex
- Armadillo like helical domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
