Cathepsin D Antibody
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Catalog number3191R-100
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PricePlease ask
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Size100 µg
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Antibody TargetCathepsin D
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Target Alternative NameCTSD , CLN10, CPSD, mgC2311
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 140 of mouse cathepsins D
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AccessionEDL18143
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Gene Id13033
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit anti-cathepsin D polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blot analysis (0.5-4 µg/ml) and in immunoprecipitation. However, the optimal conditions should be determined individually. The purified antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.
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DescriptionCathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence sμggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GeneCTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
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French translationanticorps
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Gene target
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Gene symbolCTSD
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Short nameCathepsin D Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameCathepsin D (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecathepsin D
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Synonyms gene
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Synonyms gene name
- cathepsin D (lysosomal aspartyl protease)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cathepsins
- Peptidase family A1
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data