Anti-Calponin-1 Rabbit Monoclonal Antibody

  • Catalog number
    A1609-50
  • Price
    Please ask
  • Size
    50 µl
  • Antibody Target
    Calponin-1
  • Target Alternative Name
    Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC
  • Antibody Type
    Monoclonal
  • Clone
    RM262
  • Species Reactivities
    Human
  • Immunogen Sequence
    A peptide corresponding to the C-terminus of human Calponin-1
  • Accession
    P51911
  • Gene Id
    1264
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
  • Purification
    Affinity purified
  • Application
    WB, IHC
  • Positive Control
    WB: mouse heart tissue lysates, ICC: HeLa cells
  • Application And Usages
    WB: 1:1000-1:2000, ICC: 1:100-1:200
  • Description
    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
  • Storage Condition
    -20°C
  • Shipping Condition
    gel pack
  • Shelf Life
    14 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-Calponin-1 Rabbit Monoclonal Antibody
  • Technique
    Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Rabbit, Rabbits
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibody toCalponin-1 production species: rabbit monoclonal (antibody to-)
  • Alternative technique
    antibodies, rabbit-anti
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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