Anti-Bromodeoxyuridine (Brdu), Biotin, (Clone: BU1/75 (ICR1)), (Rat IgG2a)

• Catalog number
  GEN520517
• Price
  Please ask
• Size
  100ug

• Also known as
  Bromodeoxyuridine (Brdu)
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Rat IgG2a
• Clone
  BU1/75 (ICR1)
• Host organism
  Rat (Rattus norvegicus)
• Species reactivity
  N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Bromodeoxyuridine (Brdu) (Reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. It also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not cross react with thymidine); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Protein G
• Form Appearance
  Biotin (Purified Ig in PBS/Glycerol (50%) + BSA (10 mg/ml))
• Concentration
  N/A
• Storage and shipping
  Store the antibody at +4 degrees Celsius. for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius. for longer periods. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided.
• Tested applications
  Immunocytochemistry (ICC)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Bromodeoxyuridine   Brdu   BU1/75   ICR1   IgG2a  
• Short name
  Anti-Bromodeoxyuridine (Brdu), , (Clone: BU1/75 (ICR1)), ( IgG2a)
• Technique
  anti-, anti, antibody to, antibodies
• Host
  Rat
• Isotype
  IgG2a
• Label
  Biotin
• Species
  Rat, Rats
• Alternative name
  Anti-Anti-Bromodeoxyuridine (Brdu), Biotin, (Clone: BU1/75 (ICR1)), (Rat IgG2a)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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