anti-Betacellulin Antibody
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Catalog number
38-179
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Price
Please ask
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Size
0.1 mg
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Gene info
Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).
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Gene names
ProbetacellulinBTC
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Antibodie s label
unlabelled serum
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography employing immobilized hBetacellulin matrix.
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Betacellulin (0.1 ng/mL), a concentration of ≤ 0.1 ug/mL of this antibody is required.ELISA:Indirect:To detect hBetacellulin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.SandwichTo detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Betacellulin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin. Western Blot:To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
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Keep at
Betacellulin antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
461653
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Ncbi nr
P35070
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Vial with antibody
Lyophilized
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NCBI GENE nr
685
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GENE symbol
BTC
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Antigene name
betacellulin
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Source
Homo sapiens
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Protein nr
P35070
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
BTC
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Short name
anti-Betacellulin Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toBetacellulin (antibody to-)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
betacellulin
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GenBank acession
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Locus
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Discovery year
1994-07-21
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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