Anti-Bcl-X Antibody (2H12)

• Catalog number
  A1438-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Bcl-X
• Target Alternative Name
  Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)
• Antibody Type
  Monoclonal
• Clone
  2H12
• Species Reactivities
  Human, Mouse, Rat, Pig
• Immunogen Sequence
  A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein
• Accession
  Q07817
• Gene Id
  598
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In 10 mM PBS with 0.05% BSA & 0.05% azide
• Purification
  Protein A/G purification
• Application
  FC, IF, IHC, WB
• Positive Control
  WB: Jurkat cell lysate, IHC: human Hodgkin's Lymphoma
• Application And Usages
  FC: 0.5-1 ug/1X106 cells, IF: 0.5-1 ug/ml, WB: 0.5-1ug/ml, IHC: 0.5-1 ug/ml
• Description
  Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Bcl-X   2H12  
• Short name
  Anti-Bcl-X Antibody (2H12)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG2a
• Alternative name
  Antibody toBcl-X (antibody to-) (2H12)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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