BARD1 Antibody

  • Catalog number
    3768-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    BARD1
  • Target Alternative Name
    BARD1, BARD-1
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human
  • Immunogen Sequence
    Synthetic peptide of human BARD1
  • Accession
    Q99728
  • Gene Id
    580
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot, Immunoprecipitation
  • Application And Usages
    Western blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa nuclear extract (Cat.# 1641-1) can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the BARD1 of human origin. Reactivity to other species has not been tested.
  • Description
    Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    BARD1  
  • Gene symbol
    BARD1
  • Short name
    BARD1 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    breast cancer 1, early onset associated RING domain 1 (antibody to-)
  • Alternative technique
    antibodies
  • Alternative to gene target
    BRCA1 associated RING domain 1, BARD1 and IDBG-80113 and ENSG00000138376 and 580, protein heterodimerization activity, nuclei, Bard1 and IDBG-164443 and ENSMUSG00000026196 and 12021, BARD1 and IDBG-643630 and ENSBTAG00000033690 and 530961
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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