Anti-Osteopontin (NT) (Clone 2F10)

  • Catalog number
    5423-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    Osteopontin
  • Target Alternative Name
    OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin.
  • Antibody Type
    Monoclonal
  • Clone
    2F10
  • Species Reactivities
    Recognizes native human Osteopontin from purified breast milk. Specific for the N-terminal fragment of full-length Osteopontin.
  • Immunogen Sequence
    Recombinant full-length human Osteopontin
  • Accession
    P10451
  • Gene Id
    6696
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    1 mg/ml
  • Formulation
    1 mg/ml in 0.15 M PBS
  • Purification
    Protein A chromatography from ascites
  • Application
    ELISA, Western Blot.
  • Positive Control
    Native Human OPN
  • Description
    Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications (PTM) including phosphorylation and glycosylation. Due to its acidic nature and PTMs, OPN runs anomalously by SDS-PAGE. Althoμgh its mass is 35kD, apparent molecular weights may range up to 75kD. In addition, OPN is subject to proteolytic modification into smaller molecular weight fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been sμggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Gene target
  • Short name
    Anti-Osteopontin (NT) (Clone 2F10)
  • Technique
    anti-, anti, antibody to, antibodies
  • Isotype
    Mouse IgG1κ
  • Alternative name
    Antibody toOsteopontin (NT) (clonality 2F10)
  • Alternative technique
    antibodies
  • Clone name
    2F10
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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