Anti-Ku70 Antibody
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Catalog numberA1210-100
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PricePlease ask
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Size100 µl
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Antibody TargetXRCC6
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Target Alternative NameG22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6
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Antibody TypePolyclonal
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Species ReactivitiesHuman, Mouse, Rat
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Immunogen SequenceKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ku70
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AccessionP12956
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Gene Id2547
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AppearanceColorless liquid
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FormLiquid
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FormulationIn 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide
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PurificationAffinity purified
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ApplicationWB
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Positive ControlWB; Hela, NIH3T3, PC12 cell lysates
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Application And UsagesWB; 1:500 – 1:2000
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DescriptionSingle-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans
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HandlingThe antibody solution should be gently mixed before use
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolATP23, XRCC6
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Short nameAnti-Ku70 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibody toKu70 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameATP23 metallopeptidase and ATP synthase assembly factor homolog
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Synonyms gene
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Synonyms gene name
- XRCC6 binding protein 1
- ATP23 metallopeptidase and ATP synthase assembly factor homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M76 metallopeptidase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameX-ray repair cross complementing 6
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Synonyms gene
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Synonyms gene name
- thyroid autoantigen 70kD (Ku antigen)
- thyroid autoantigen 70kDa (Ku antigen)
- X-ray repair complementing defective repair in Chinese hamster cells 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA helicases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data