Anti-Flavivirus group antigen Antibody

• Catalog number
  A1146-200
• Price
  Please ask
• Size
  200 µg

• Antibody Type
  Monoclonal
• Clone
  D1-4G2-4-15 (4G2)
• Immunogen Sequence
  Dengue Virus type 2 antigens
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  200 µg affinity purified antibody in phosphate-buffered saline (PBS) containing 0.02% Proclin 300
• Purification
  Affinity purified using protein A
• Application
  IF; WB; NTRL; ELISA
• Positive Control
  IF: Vero cells infected with ZIKV
• Description
  This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation. The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004. Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008).
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Flavivirus   group  
• Short name
  Anti-Flavivirus group antigen Antibody
• Technique
  Antibody, antigen, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antigenes
• Isotype
  Rabbit IgG, kappa
• Alternative name
  Antibody toFlavivirus family protein (antibody to-)
• Alternative technique
  antibodies, antigenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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