Anti-CD16/CD32, Rabbit IgG Antibody
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Catalog number
A1107-200
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Price
Please ask
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Size
200 µg
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Antibody Type
Monoclonal
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Clone
2.4G2
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Species Reactivities
Mouse
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Immunogen Sequence
Mouse BALB/c Macrophage J774 Cell Line.
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
200 µg affinity purified human antibody in phosphate-buffered saline (PBS) containing 0.02% Proclin 300
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Purification
Affinity purified using protein A
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Application
FC; IF; IP; IHC; Block
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Description
Recombinant monoclonal antibody to CD16/CD32. Manufactured using recombinant technology with variable regions (i.e. specificity) from the hybridoma 2.4G2. This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
FCGR3A, FCGR2B, FCGR3B, FCGR2A
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Short name
Anti-CD16/CD32, Rabbit IgG Antibody
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Technique
Antibody, Rabbit, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, IgGs
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Host
Rabbit, Rabbits
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Isotype
Rabbit IgG, kappa, IgG
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Alternative name
Antibody toCD16/CD32, production species: rabbit Immunoglobulin G (antibody to-)
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Alternative technique
antibodies, rabbit-anti, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIIa
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
- Fc fragment of IgG receptor IIIa
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Synonyms
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GenBank acession
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Locus
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Discovery year
1988-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIb
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIb, receptor for (CD32)
- Fc fragment of IgG, low affinity IIb, receptor (CD32)
- Fc fragment of IgG receptor IIb
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Synonyms
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GenBank acession
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Locus
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Discovery year
1991-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
Fc gamma receptor IIIb
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Synonyms gene
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Synonyms gene name
- Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
- Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
- Fc fragment of IgG receptor IIIb
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Synonyms
-
GenBank acession
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Locus
-
Discovery year
1991-08-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene name
Fc gamma receptor IIa
-
Synonyms gene
-
Synonyms gene name
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG receptor IIa
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1988-11-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Immunoglobulin like domain containing
- CD molecules
-
VEGA ID
-
Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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