Phospho-Akt2 (Ser474) Antibody
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Catalog number
A1246-100
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Price
Please ask
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Size
100 µl
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Antibody Target
AKT2
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Target Alternative Name
AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
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Antibody Type
Polyclonal
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Species Reactivities
Human, mouse , rat
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Immunogen Sequence
Synthesized peptide derived from human Akt2 around the phosphorylation site of S474
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Accession
P31751
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Gene Id
208
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
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Purification
Affinity-chromatography using epitope-specific immunogen
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Application
WB, ELISA, IHC
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Positive Control
WB: Mouse brain lysate, 293 cell lysate
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Application And Usages
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000
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Description
AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Phospho-Akt2 (Ser474) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
phosphorilated-v-akt murine thymoma viral oncogene homolog 2 (Ser474) (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
v-akt murine thymoma viral oncogene homolog 2, HIHGHH and PKBB and PKBBETA and PRKBB and RAC-BETA, AKT2 and IDBG-51270 and ENSG00000105221 and 208, transferase activity, nuclei, Akt2 and IDBG-161456 and ENSMUSG00000004056 and 11652, AKT2 and IDBG-637808 and ENSBTAG00000001400 and 534923
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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