ADAM10 Antibody

• Catalog number
  3201-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  ADAM10
• Target Alternative Name
  MADM , CDw156, CD156c , kuz , EC 3.4.24.81, HsT18717
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Synthetic peptide corresponding to residue surrounding amino acid 735 of human ADAM10
• Accession
  AAC51766
• Gene Id
  2393947
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-ADAM10 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (1-4 µg/ml) and immunofluorescence (2-10 µg/ml). An 85 kDa band corresponding to the precursor form of ADAM10 can be detected. Jurkat and K562 cell lysates can be used as positive controls. However, the optimal conditions should be determined individually.
• Description
  Proinflammatory cytokine tumor necrosis factorα (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ADAM10  
• Gene symbol
  ADAM10
• Short name
  ADAM10 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  ADAM metallopeptidase domain 10 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  ADAM metallopeptidase domain 10, AD10 and AD18 and CD156c and HsT18717 and kuz and MADM and RAK, ADAM10 and IDBG-14155 and ENSG00000137845 and 102, protein homodimerization activity, nuclei, Adam10 and IDBG-182594 and ENSMUSG00000054693 and 11487, ADAM10 and IDBG-646594 and ENSBTAG00000005481 and 282132

Gene info

• Identity
  HGNC:188
• Gene
  ADAM10
• Long gene name
  ADAM metallopeptidase domain 10
• Synonyms gene name
  • a disintegrin and metalloproteinase domain 10
• Synonyms
  • kuz
  • MADM
  • HsT18717
  • CD156C
• GenBank acession
  • AF009615
• Locus
  15q21.3
• Discovery year
  1997-03-21
• Entrez gene record
  102
• Pubmed identfication
  • 9344679
  • 9441766
• RefSeq identity
  • NM_001110
• Classification
  • ADAM metallopeptidase domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000132633

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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