Anti-ARP2/ARP3 (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-12524R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideARP2/ARP3
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SpecificityThis antibody reacts specifically with ARP2/ARP3
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human ARP2/ARP3
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Gene ID10097
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationActin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsActin like protein 2; Actin related protein 2; Actin-like protein 2; ACTIN-RELATED PROTEIN 2; ACTR2; ARP2 actin related protein 2 homolog yeast; ARP2; ARP2/3 COMPLEX; ARP2_HUMAN; OTTHUMP00000159937; OTTHUMP00000202270.
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Also known asARP2/ARP3 Polyclonal Antibody
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Other nameAnti-ARP2/ARP3 Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolACTR3, ACTR2, APOBEC3A, ANGPTL2, AP4M1, AICDA
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Short nameAnti-ARP2/ARP3
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameARP2/ARP3 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameactin related protein 3
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Synonyms gene name
- ARP3 (actin-related protein 3, yeast) homolog
- ARP3 actin-related protein 3 homolog (yeast)
- ARP3 actin related protein 3 homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related protein 2/3 complex subunits
- Actin related proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactin related protein 2
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Synonyms gene name
- ARP2 (actin-related protein 2, yeast) homolog
- ARP2 actin-related protein 2 homolog (yeast)
- ARP2 actin related protein 2 homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-05-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related protein 2/3 complex subunits
- Actin related proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameapolipoprotein B mRNA editing enzyme catalytic subunit 3A
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Synonyms gene name
- apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-12-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Apolipoprotein B mRNA editing enzyme catalytic subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameangiopoietin like 2
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Synonyms gene name
- angiopoietin-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-02-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Angiopoietin like family
- Fibrinogen C domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameadaptor related protein complex 4 subunit mu 1
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Synonyms gene name
- adaptor related protein complex 4 mu 1 subunit
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Adaptor related protein complex 4
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameactivation induced cytidine deaminase
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Synonyms gene name
- activation-induced cytidine deaminase
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-09-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Apolipoprotein B mRNA editing enzyme catalytic subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data