anti-5 Lipoxygenase (phospho Ser523) Antibody
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Catalog number
50-244
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Price
Please ask
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Size
0.1 mL
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Gene info
The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.
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Gene names
5-LO, 5LPG, LOG5, 5-LOX,
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-lipoxygenase (5-LO).
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Immuno application
Western Blot
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Antibody advice
The antibody has been directly tested for reactivity in Western blots with rat and human tissue. It is anticipated that the antibody will react with non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
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Suspention pH azide
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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Keep at
5 Lipoxygenase antibody can be stored at -20 grades C and is stable at -20 grades C for at least 1 year.
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Works with
Human, Rat
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Omim nr
126407
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Ncbi nr
P09917
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KDa
80
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Vial with antibody
in solution
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NCBI GENE nr
240
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GENE symbol
ALOX5
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Antigene name
arachidonate 5-lipoxygenase
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Source
Homo sapiens
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Protein nr
P09917
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
anti-5 Lipoxygenase (phospho Ser523) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody to5 Lipoxygenase (phosphorilated Ser523) (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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