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Summary
• Detection method - Fluorescence (Ex/Em = 485/530 nm) • Applications: Measure cell anoikis in response to stimuli. - Screen, study, or characterize compounds that influence Anoikis. - HTP adaptable
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Detection Method
Fluorescence (Ex/Em = 485/530 nm)
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Species Reactivity
Mammalian cells
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Applications
• Measure cell anoikis in response to stimuli. - Screen, study, or characterize compounds that influence Anoikis. - HTP adaptable
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Sample Type
• Metastatic cell lines • Anoikis inhibitor or stimuli
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Features Benefits
• Highly sensitive fluorometric method to measure cell Anoikis in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Anoikis
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Anoikis is a form of cellular apoptosis induced by the inability of normal cells to attach to the extracellular matrix (ECM). Incorrect attachment or no ECM attachment could affect cell growth and differentiation in normal cells. However, research studies has determined that metastatic cells can undergo Anoikis without affecting their ability to migrate and invade surrounding or distant organs. BioVision’s Anoikis assay kit quantifies live cells using fluorometric (Calcein AM) reagents and a proprietary coated chamber that mimics Anoikis conditions. Our assay is easy to use, sensitive and compatible with fluorimeters
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Test
Biovision supplies other types of Assays as 1.
