Annexin V-Biotin Apoptosis Kit

  • Catalog number
    K109-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Detects Apoptosis by FACS or FL
  • Summary
    • Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Sample Type
    Living cells (suspension and adherent)
  • Features Benefits
    • Simple one step staining procedure in 30 minutes • Fast and convenient • Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
  • Storage Conditions
    +4°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
  • Additional description
    This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
    Annexin   Kit  
  • Short name
    Annexin V- Apoptosis Kit
  • Technique
    apoptosis, apoptosis pathway
  • Label
    Biotin
  • Alternative name
    Annexin V-biotinilated programmed cellular destruction reagent
  • Alternative technique
    celldeath, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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