Amplite Colorimetric Biotin Quantitation Kit

  • Catalog number
    5522
  • Price
    Please ask
  • Size
    200 Tests
  • Description
    Amplite Colorimetric Biotin Quantitation Kit is used for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
  • Shipping
    Room Temperature
  • Storage
    Freezer
  • Shelflife
    6 months
  • Kit Components
    Component A: avidinĀ ; Component B: HABA Assay Buffer; Component C: D-Biotin
  • Ex nm
    500 nm
  • Em nm
    NA
  • Personal Protection
    Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
  • Uses
    Laboratory chemicals *For Research Use Only*
  • Ordering
    To order Amplite Colorimetric Biotin Quantitation Kit, please use the Cat. Nr.5522 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Biotin Quantitation Kit
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Colorimetric assays or detection use UV absorption or enzymatic color reaction.
  • Conjugation
    Biotinylated
  • Gene target
  • Short name
    Amplite Colorimetric Quantitation Kit
  • Technique
    colorimetric, colorimetry
  • Label
    Biotin
  • Alternative name
    Amplite colorimetry biotinilated Quantitation reagent
  • Alternative technique
    assays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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