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Stock availability
In Stock
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Kit s description
Colorimetric detection of alpha B Crystallin
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Protein target
alpha B Crystallin
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Scientific context
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. alpha and beta families are further divided into acidic and basic groups (alpha-A and alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
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Primary research area
Cancer, Heat Shock
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Category
Assay ELISA Kits
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Other name
AACRYA ELISA Kit, CRYA2 ELISA Kit, CRYAB ELISA Kit, CTPP2 ELISA Kit, HSPB5 ELISA Kit, NY Ren 27 antigen ELISA Kit, alpha crystallin B ELISA Kit
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Brand name
StressXpress®
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Detection system
Colorimetric Assay
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Assay format
Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
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Main intended usage
ELISA kit used to quantitate alpha B crystallin concentration in samples.
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Brief protocol
1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
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Kit contents
Anti-alpha B Crystallin Immunoassay Plate, 5X alpha B Crystallin Extraction Reagent, Recombinant alpha B Crystallin Standard, Standard and Sample Diluent, 10X Wash Buffer Concentrate, Anti-alpha B Crystallin Biotinylated Antibody Concentrate, Anti-alpha B Crystallin Biotinylated Antibody Diluent, Streptavidin: HRP Concentrate, Streptavidin: HRP Diluent, TMB Substrate, Stop Solution
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Assay precision
Contact us
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Assay cross reactivity
Human
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Samples to be used with this kit
Cell Lysates, Tissue, Serum
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Maximum samples to be used with this kit
40 samples in duplicate
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Assay duration
30 minutes
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Kit s sensitivity
0,009 ng/ml
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Assay detection limit
0,352 - 22,5 ng/ml
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Storage recommendations
4°C
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Shipping recommendations
Blue Ice
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Bibliography
1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.
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Release date
2-Feb-2011
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PubMed number
25311653
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Tested applications
ELISA
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Tested reactivity
Human
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Resources available upon request
Kit Booklet, MSDS
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Product image legend
Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-123. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.352 - 22.5 ng/mL. Graph of the Typical Standard Curve for the Alpha B Crystallin ELISA Kit StressXpress - SKT-123
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Warnings
Non-hazardous
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Country of production
Canada
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Total weight kg
2
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Net weight g
2500
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Description
The Alpha B Crystallin ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
CRYAB, HSPB9, HSPB6
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Short name
Alpha B Crystallin ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
a B Crystallin Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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