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Available ordering format
Lyophilized in phosphate buffer saline
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Immunogen
native alpha-mannosidase purified from jack beans
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Purified IgG
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How to reconstitute
For reconstitution please add 1 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
AS09 452 | anti-alpha-mannosidase (total IgG)AS09 650 | anti-alpha-mannosidase (affinity purified)Plant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 - 1: 100 000 for techniques listed above
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Canavalia ensiformis (common jack-bean)
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Possible reactivity
n.a.
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Biotin/IgG protein molar ration is approximately 1.4. No foreign proteins are added.
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References
to be added when available
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Scientific context
Alpha-mannosidase cleaves the alpha form of mannose.
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Notes
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
The Alpha, Alpha-mannosidase, conjugated is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
