Alpha A Crystallin Antibody / CRYAA
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Catalog number
R32408
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Alpha A Crystallin / CRYAA
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P
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Recommended dilutions
Western blot: 0.1-0.5ug/ml,IHC (FFPE): 0.5-1ug/ml
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Notes
Optimal dilution of the Alpha A Crystallin antibody should be determined by the researcher.
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Intented use
This Alpha A Crystallin antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P02489
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Purity
Antigen affinity
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Description
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
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Immunogen
Amino acids M1-S173 from the human protein were used as the immunogen for the Alpha A Crystallin antibody.
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Storage
After reconstitution, the Alpha A Crystallin antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Localization
Nuclear, cytoplasmic
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Additional description
The Anti-Alpha A Crystallin / CRYAA is a α- or alpha protein sometimes glycoprotein present in blood.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
CRYAA
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Short name
Anti-Alpha A Crystallin / CRYAA
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to Alpha A Crystallin / CRYAA
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Alternative technique
antibodies
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Alternative to gene target
crystallin, alpha A, CRYAA and IDBG-4699 and ENSG00000160202 and 102724652,1409, unfolded protein binding, nuclei, Cryaa and IDBG-164758 and ENSMUSG00000024041 and 12954, CRYAA and IDBG-639603 and ENSBTAG00000003134 and 281718
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Gene info
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Identity
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Gene
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Long gene name
crystallin alpha A
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Synonyms gene
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Classification
- Small heat shock proteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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