Aldose Reductase, human recombinant

• Catalog number
  7361-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR
• Alternative_names
  Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR
• Description
  Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is approximately 0.2-0.9 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/min at 25°C. Specific activity was expressed in units/mg of protein.
• Molecular Weight
  35.8 kDa (316 aa, 1-316 aa), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
• Background Information
  Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Aldose   Reductase  
• Gene symbol
  AKR1B10, AKR1B1
• Short name
  Aldose Reductase, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Aldose Reductase, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:382
• Gene
  AKR1B10
• Long gene name
  aldo-keto reductase family 1 member B10
• Synonyms gene
  • AKR1B11
• Synonyms gene name
  • aldo-keto reductase family 1, member B10 (aldose reductase)
• Synonyms
  • AKR1B12
  • ARL-1
  • HIS
  • ARL1
  • HSI
  • ALDRLn
• Synonyms name
  • aldose reductase-like 1
  • aldo-keto reductase family 1, member B11 (aldose reductase-like)
  • aldose reductase-like peptide
  • aldose reductase-related protein
  • small intestine reductase
• GenBank acession
  • AF052577
• Locus
  7q33
• Discovery year
  2000-08-24
• Entrez gene record
  57016
• Pubmed identfication
  • 9765596
  • 9565553
• RefSeq identity
  • NM_020299
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000155356

Gene info

• Identity
  HGNC:381
• Gene
  AKR1B1
• Long gene name
  aldo-keto reductase family 1 member B
• Synonyms gene
  • ALDR1
• Synonyms gene name
  • aldo-keto reductase family 1, member B1 (aldose reductase)
• Synonyms
  • AR
• Synonyms name
  • aldose reductase
• GenBank acession
  • J04795
• Locus
  7q33
• Discovery year
  1991-08-07
• Entrez gene record
  231
• Pubmed identfication
  • 1901827
• RefSeq identity
  • NM_001628
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000155322

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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