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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Proteases, Inhibitors, & Enzymes
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Reactivity
Human
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Residues
1-453 amino acids: MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
0.5 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is < 1 units/ml and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 uM of acetaldehyde to acetic acid per minute at pH 8.0 at 25 DEG C in the presence of beta-NAD, potassium and thiols.
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Assay Information
1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 2.5ug of recombinant ALDH3A1 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes
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Additional Information
ALDH3A1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugation
histidine
