HIS Tag (6AT18) Monoclonal Antibody

  • Catalog number
    bsm-51006M
  • Price
    Please ask
  • Size
    0.1ml
  • Long name
    HIS Tag (6AT18) Monoclonal Antibody
  • Also known as
    HIS Tag (6AT18) Monoclonal Antibody
  • Category
    Primary Antibodies
  • Conjugation
    Unconjugated
  • Target Antigen
    HIS Tag
  • Specificity
    This is a highly specific antibody against HIS Tag.
  • Modification s
    None
  • Modification site s
    Unmodified antibody
  • Clonality
    Monoclonal
  • Clone number
    6AT18
  • Source
    Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
  • Tested Applications
    WB
  • Recommended dilutions
    WB(1:1000-2000)
  • Cross reactivity
    Others
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the target antigen
    Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
  • Purification method
    Mouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Storage conditions
    Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
  • Properties
    If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Additional conjugation
    histidine
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
    HIS   Tag   6AT18   Monoclonal  
  • Gene symbol
    HAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
  • Short name
    Anti-HIS Tag
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
  • Host
    Mouse (Mus musculus)
  • Isotype
    Immunoglobulin G (IgG)1
  • Label
    His
  • Alternative name
    HIS Tag (6AT18) Monoclonal Antibody
  • Alternative technique
    antibodies
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 4 (anticodon GUG)
    • transfer RNA-His (GTG) 1-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-21
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-3
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 9 (anticodon GUG)
    • transfer RNA-His (GTG) 1-3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (GTG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 11 (anticodon GUG)
    • transfer RNA-His (GTG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
    1
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Low confidence cytoplasmic transfer RNAs
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-2
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 10 (anticodon GUG)
    • transfer RNA-His (GTG) 1-2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-4
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 7 (anticodon GUG)
    • transfer RNA-His (GTG) 1-4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 3 (anticodon GUG)
    • transfer RNA-His (GTG) 1-8
    • transfer RNA-His (GTG) 1-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-09-03
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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