HIS Tag (6AT18) Monoclonal Antibody
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Catalog numberbsm-51006M
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PricePlease ask
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Size0.1ml
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Long nameHIS Tag (6AT18) Monoclonal Antibody
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Also known asHIS Tag (6AT18) Monoclonal Antibody
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenHIS Tag
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SpecificityThis is a highly specific antibody against HIS Tag.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityMonoclonal
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Clone number6AT18
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SourcePurified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
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Tested ApplicationsWB
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Recommended dilutionsWB(1:1000-2000)
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Cross reactivityOthers
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenEpitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
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Purification methodMouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional conjugationhistidine
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolHAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
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Short nameAnti-HIS Tag
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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HostMouse (Mus musculus)
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IsotypeImmunoglobulin G (IgG)1
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LabelHis
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Alternative nameHIS Tag (6AT18) Monoclonal Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namehistidine ammonia-lyase
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Synonyms gene
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Locus
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Discovery year1988-08-31
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameviral integration site 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-25
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namealdo-keto reductase family 1 member B10
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Synonyms gene
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Synonyms gene name
- aldo-keto reductase family 1, member B10 (aldose reductase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aldo-keto reductases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 4 (anticodon GUG)
- transfer RNA-His (GTG) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-21
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 9 (anticodon GUG)
- transfer RNA-His (GTG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (GTG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 11 (anticodon GUG)
- transfer RNA-His (GTG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 8 (anticodon GUG)
- transfer RNA-His (GTG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-2
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 10 (anticodon GUG)
- transfer RNA-His (GTG) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 7 (anticodon GUG)
- transfer RNA-His (GTG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 3 (anticodon GUG)
- transfer RNA-His (GTG) 1-8
- transfer RNA-His (GTG) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-09-03
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data