Affinity purified Rat IgG1 Isotype Antibody PerCP-Conjugated
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Catalog number
ERIS005-100
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Price
Please ask
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Size
100 mg
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Clone
HRPN
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Source
Rat
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Clonality
Monoclonal
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Category
Isotype Control
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Description
Rat IgG1 was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
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Reactivity
Not specified / Inquire
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Conjugation
PerCP
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Purification
Affinity purified
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Antibody s supplied with
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
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Antibody s concentration
0,5 mg/ml
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Storage
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
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Antibody s is used for
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
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Test
A high affinity purification column was use to purify IgG1 Isotype Antibody PerCP-Conjugated by Elabscience by chromatographic size exclusion.
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Additional description
1 of protein A or G purified by Elabscience will give more specificity than crude serum.
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Properties
If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Group
isotypes
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Conjugate
Peridinin chlorophyll (PerCP)
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Gene target
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Short name
IgG1 Isotype Antibody PerCP-Conjugated
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Technique
affinity, Antibody, purified, Isotype, Percp, antibodies against human proteins, antibodies for, antibody Conjugates, Isotypes and controls, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the IgG1 Isotype Antibody PerCP-Conjugated is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Elabscience. purifications
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Host
Rat
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Isotype
IgG1, IgG1
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Label
PerCP
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Species
Rat, Rats
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Alternative name
protein enriched Rat IgG1 isoform (antibody to-) PerCP-coupled
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Alternative technique
pure, antibodies, control, antibodies-percp
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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