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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At2g17130
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS04 054 | anti-AOX1/2 rabbit antibody, marker of inner mitochondrial membraneAS04 053A | anti-COXII rabbit antibody marker of inner mitochondrial membraneAS07 212 | anti-VDAC1 rabbit antibody marker of outer mitochondrial membrane
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Recommended dilutions for use
1 : 5 000 with standard ECL (WB)
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Molecular weight expected аpparent
39 | 45 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Capsicum annuum, Lycopersicum chilense, Nicotiana benthamiana, Solanum lycopersicum , Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
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Possible reactivity
Brachypodium distachyon, Brassica napus, Capsella rubella, Citrus sinensis, Glycine max, Hordeum vulgare, Malus x domestica, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
cellular [compartment marker] of mitochondrial matrix
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References
Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005. Choi et al. (2014). Pepper Mitochondrial FORMATE DEHYDROGENASE1 Regulates Cell Death and Defense Responses against Bacterial Pathogens. Plant Physiol. 2014 Sep 18. pii: pp.114.246736. Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144. Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7.
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Scientific context
Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.
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Notes
Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits. Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form).
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Protein number
Q8LFCO , P93032
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TAIR number
AT4G35260
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