Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

  • Catalog number
    K807-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Only kit on the market to measure AHCY activity
  • Summary
    • Detection Method – Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of AHCY activity
  • Detection Method
    Fluorescence (Ex/Em = 535/587 nm)
  • Applications
    Detection of AHCY activity
  • Sample Type
    • Purified recombinant protein • Cell and tissue lysate
  • Features Benefits
    • Rapid, simple & convenient • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
  • Gene symbol
    AHCY
  • Short name
    Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    Adenosylhomocysteinase (AHCY) Activity fluorescence test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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