Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
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Catalog number
K807-100
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Price
Please ask
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Size
100 assays
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Description
Only kit on the market to measure AHCY activity
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Summary
• Detection Method – Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of AHCY activity
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Detection Method
Fluorescence (Ex/Em = 535/587 nm)
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Applications
Detection of AHCY activity
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Sample Type
• Purified recombinant protein • Cell and tissue lysate
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Features Benefits
• Rapid, simple & convenient • The assay can detect AHCY activity as low as 1 µU recombinant human AHCY.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
AHCY
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Short name
Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
Adenosylhomocysteinase (AHCY) Activity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
adenosylhomocysteinase
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Synonyms gene name
- S-adenosylhomocysteine hydrolase
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Synonyms
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Product images
Product files
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