b-Actin Antibody

• Catalog number
  R30971
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  b-Actin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This b-Actin antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P60709
• Purity
  Antigen affinity
• Description
  b-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.
• Immunogen
  Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the b-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  b-Actin  
• Gene symbol
  ACTR3B, ACTR1B, FLNB
• Short name
  Anti-b-Actin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to b-Actin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17256
• Gene
  ACTR3B
• Long gene name
  actin related protein 3B
• Synonyms gene name
  • ARP3 actin-related protein 3 homolog B (yeast)
  • ARP3 actin related protein 3 homolog B
• Synonyms
  • ARP11
  • ARP3beta
• Locus
  7q36.1-q36.2
• Discovery year
  2005-05-19
• Entrez gene record
  57180
• Pubmed identfication
  • 10806390
• RefSeq identity
  • NM_020445
• Classification
  • Actin related proteins
• VEGA ID
  OTTHUMG00000151463

Gene info

• Identity
  HGNC:168
• Gene
  ACTR1B
• Long gene name
  actin related protein 1B
• Synonyms gene
  • CTRN2
• Synonyms gene name
  • ARP1 (actin-related protein 1, yeast) homolog B (centractin beta)
  • ARP1 actin-related protein 1 homolog B, centractin beta
  • ARP1 actin related protein 1 homolog B
• Synonyms
  • Arp1B
• Synonyms name
  • centractin beta
• GenBank acession
  • X82207
• Locus
  2q11.2
• Discovery year
  1999-05-17
• Entrez gene record
  10120
• Pubmed identfication
  • 7696711
  • 10343100
• RefSeq identity
  • NM_005735
• Classification
  • Actin related proteins
• VEGA ID
  OTTHUMG00000130549

Gene info

• Identity
  HGNC:3755
• Gene
  FLNB
• Long gene name
  filamin B
• Synonyms gene
  • FLN1L
  • LRS1
• Synonyms gene name
  • filamin B, beta (actin binding protein 278)
  • Larsen syndrome 1 (autosomal dominant)
  • filamin B, beta
• Synonyms
  • TAP
  • TABP
  • ABP-278
  • FH1
• Synonyms name
  • actin binding protein 278
  • beta filamin
• GenBank acession
  • AF043045
• Locus
  3p14.3
• Discovery year
  1997-06-20
• Entrez gene record
  2317
• Pubmed identfication
  • 8327473
  • 10449914
  • 14991055
  • 16801345
• RefSeq identity
  • NM_001457
• Classification
  • Filamins
• VEGA ID
  OTTHUMG00000159158

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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