ACTN4 Antibody / Alpha Actinin 4

• Catalog number
  R32459
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ACTN4 / Alpha Actinin 4
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This ACTN4 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O43707
• Purity
  Antigen affinity
• Description
  Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
• Immunogen
  Amino acids E561-V661 from the human protein were used as the immunogen for the ACTN4 antibody.
• Storage
  Prior to reconstitution, store at 4oC. After reconstitution, the ACTN4 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic
• Additional description
  The Anti- ACTN4 / Alpha Actinin 4 is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ACTN4   Alpha   Actinin  
• Gene symbol
  ACTN4
• Short name
  Anti- ACTN4 / Alpha Actinin 4
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to ACTN4 / Alpha Actinin 4
• Alternative technique
  antibodies
• Alternative to gene target
  actinin, alpha 4, ACTININ-4 and FSGS and FSGS1, ACTN4 and IDBG-49384 and ENSG00000130402 and 81, actin filament binding, nuclei, Actn4 and IDBG-165033 and ENSMUSG00000054808 and 60595, ACTN4 and IDBG-637203 and ENSBTAG00000011215 and 522269

Gene info

• Identity
  HGNC:166
• Gene
  ACTN4
• Long gene name
  actinin alpha 4
• Synonyms gene
  • FSGS1
• Synonyms gene name
  • focal segmental glomerulosclerosis 1
• GenBank acession
  • D89980
• Locus
  19q13.2
• Discovery year
  1992-03-26
• Entrez gene record
  81
• Pubmed identfication
  • 9461087
  • 10700177
• Classification
  • Actinins
• VEGA ID
  OTTHUMG00000137382

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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