Active Sonic Hedgehog (SHH), human recombinant
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Catalog number4010-1000
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PricePlease ask
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Size1 mg
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SynonymsSHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
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Alternative_namesSHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
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DescriptionHedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥97%
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AssaySDS-PAGE
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Purity≥97%
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Biological activityHuman recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED₅₀, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
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Results750ng/ml.
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Molecular Weight19.697kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form descriptionLyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
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Reconstitution InstructionsWhen reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
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Background InformationRecombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSHH
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Short nameActive Sonic Hedgehog (SHH), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namefunctionnal Sonic Hedgehog (sonic hedgehog), H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetsonic hedgehog, HHG1 and HLP3 and HPE3 and MCOPCB5 and SMMCI and TPT and TPTPS, SHH and IDBG-50464 and ENSG00000164690 and 6469, laminin-1 binding, nuclei, Shh and IDBG-144309 and ENSMUSG00000002633 and 20423, SHH and IDBG-638249 and ENSBTAG00000024552 and
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Gene info
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Identity
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Gene
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Long gene namesonic hedgehog signaling molecule
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Synonyms gene
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Synonyms gene name
- sonic hedgehog (Drosophila) homolog
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Synonyms
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Locus
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Discovery year1995-03-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hedgehog signaling molecule family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data