Active HRV14 3C Protease Recombinant

• Catalog number
  8012-100
• Price
  Please ask
• Size
  100 µg

• Synonyms
  Precission protease, 3C Protease
• Alternative_names
  Precission protease, 3C Protease
• Description
  ≥90% Pure Active Recombinant HRV14 3C Protease for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥ 90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
• Biological activity
  BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
• Results
  BioVision’s Active HRV14 3C Protease has been tested for its activity using HRV 3C Protease Activity Assay Kit (Colorimetric) (K214-100).
• Molecular Weight
  20 kDa (1538-1719 aa).
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  6 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 50 mM Tris, 100 mM NaCl, 5 mM DTT, pH 8.0 containing 20% glycerol
• Background Information
  Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s active HRV14 3C Protease is a recombinant form of the 3C protease from human rhinovirus type 14. The enzyme is highly pure with selective and high site-specific cleavage activity for Precission cleavage site (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro), thereby allowing for the production of recombinant proteins with minimal number of extra amino acids. HRV14 3C Protease also has therapeutic implications as a potential target for the development of antiviral agents.
• Amino acid sequence
  1538-1719 aa
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   HRV14   Protease  
• Short name
  Active HRV14 3C Protease Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  functionnal HRV14 3C Protease Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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