Active HIV1 Protease Recombinant (GST-tagged)

• Catalog number
  7849-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
• Alternative_names
  HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
• Description
  ≥90% Pure Active Recombinant HIV1 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  BioVision’s Active HIV1 Protease has been tested for its activity using HIV1 Protease Activity Assay Kit (K825-100).
• Molecular Weight
  38.4 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31 kDa during SEC and SDS-PAGE analyses.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  6 months
• Concentration
  3 mg/ml
• Appearance
  Liquid
• Physical form description
  3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol
• Background Information
  HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells.
• Amino acid sequence
  1-99 aa + N-terminal GST and C-terminal Poly-his tags
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   HIV1   Protease   GST-tagged  
• Gene symbol
  MGST3
• Short name
  Active HIV1 Protease Recombinant (GST-tagged)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  functionnal HIV1 Protease Rec. (GST-tagged)
• Alternative technique
  rec
• Virus
  hiv1

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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