Active HIV1 Protease Recombinant (GST-tagged)
-
Catalog number
7849-100
-
Price
Please ask
-
Size
100 ug
-
-
Synonyms
HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
-
Alternative_names
HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
-
Description
≥90% Pure Active Recombinant HIV1 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
-
Recombinant
Yes
-
Source
E. coli
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Biological activity
BioVision’s Active HIV1 Protease has been tested for its activity using HIV1 Protease Activity Assay Kit (K825-100).
-
Molecular Weight
38.4 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31 kDa during SEC and SDS-PAGE analyses.
-
Storage Temp
-80°C
-
Shipping
Dry Ice
-
Shelf Life
6 months
-
Concentration
3 mg/ml
-
Appearance
Liquid
-
Physical form description
3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol
-
Background Information
HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells.
-
Amino acid sequence
1-99 aa + N-terminal GST and C-terminal Poly-his tags
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
MGST3
-
Short name
Active HIV1 Protease Recombinant (GST-tagged)
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative name
functionnal HIV1 Protease Rec. (GST-tagged)
-
Alternative technique
rec
-
Virus
hiv1
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products