Active Caspase-1, rat recombinant

• Catalog number
  7524-25
• Price
  Please ask
• Size
  25 units

• Synonyms
  Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
• Alternative_names
  Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.
• Description
  A cysteine protease involved in apoptosis
• Recombinant
  Yes
• Source
  E. coli
• Biological activity
  The caspase-1 is routinely tested at BioVision for its ability to cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).
• Results
  >3000 units/mg
• Molecular Weight
  ~ 34.6 KDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Semi-Dry
• Physical form description
  Semi-Dry
• Reconstitution Instructions
  Reconstitute to 1 unit per ul in PBS containing 15% glycerol.
• Background Information
  Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   Caspase-1  
• Short name
  Active Caspase-1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  functionnal caspase-1, rat Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

Product images

Similar products