beta-Catenin Antibody

• Catalog number
  V2103SAF-100UG
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  1 mg/ml in 1X PBS; BSA free, sodium azide free
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  beta-Catenin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) and Mouse (Mus musculus). Other species not known. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, FACS, IF, IHC
• Recommended dilutions
  FACS: 0.5-1ug/million cells,IF: 1-2ug/ml,WB: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml for 30 minutes at RT (1)
• Notes
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Added buffer
  1X Phosphate Buffered Saline (PBS), pH 7.4
• Intented use
  This beta-Catenin antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  1499
• Purity
  Purified by protein A Affinity Chromatography
• Description
  Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
• Immunogen
  A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.
• Storage
  Store the beta-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Localization
  Cell surface and cytoplasmic
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  beta-Catenin  
• Gene symbol
  CTNNBIP1, CTNNB1, DACT1
• Short name
  Anti-beta-Catenin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to beta-Catenin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16913
• Gene
  CTNNBIP1
• Long gene name
  catenin beta interacting protein 1
• Synonyms gene name
  • catenin, beta-interacting protein 1
  • catenin, beta interacting protein 1
• Synonyms
  • ICAT
  • MGC15093
• Synonyms name
  • beta-catenin-interacting protein ICAT
  • inhibitor of beta-catenin and Tcf-4
• GenBank acession
  • AB021262
• Locus
  1p36.22
• Discovery year
  2001-11-14
• Entrez gene record
  56998
• Pubmed identfication
  • 10898789
• RefSeq identity
  • NM_020248
• VEGA ID
  OTTHUMG00000001796

Gene info

• Identity
  HGNC:2514
• Gene
  CTNNB1
• Long gene name
  catenin beta 1
• Synonyms gene
  • CTNNB
• Synonyms gene name
  • catenin (cadherin-associated protein), beta 1 (88kD)
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin (cadherin-associated protein), beta 1
• Synonyms
  • beta-catenin
  • armadillo
• GenBank acession
  • X87838
• Locus
  3p22.1
• Discovery year
  1993-07-13
• Entrez gene record
  1499
• Pubmed identfication
  • 7829088
• RefSeq identity
  • NM_001098210
• Classification
  • Beta catenins
  • Wnt enhanceosome complex
  • Armadillo repeat containing
• VEGA ID
  OTTHUMG00000131393
• Locus Specific Databases
  • LRG_1108

Gene info

• Identity
  HGNC:17748
• Gene
  DACT1
• Long gene name
  dishevelled binding antagonist of beta catenin 1
• Synonyms gene name
  • dapper homolog 1, antagonist of beta-catenin (xenopus)
  • dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis)
• Synonyms
  • DAPPER1
  • THYEX3
  • HDPR1
  • DAPPER
  • FRODO
• GenBank acession
  • AF251079
• Locus
  14q23.1
• Discovery year
  2003-01-15
• Entrez gene record
  51339
• Pubmed identfication
  • 11970895
• RefSeq identity
  • NM_016651
• Classification
  • dishevelled binding antagonist of beta catenin family
• VEGA ID
  OTTHUMG00000140324

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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