ACT, Actin (monoclonal)
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Catalog numberAS16 3141
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PricePlease ask
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Size100 µg
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Available ordering formatInquire
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ImmunogenFull length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293
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Raised inMouse
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ClonalityMonoclonal
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CloneIgG2b, clone mAbGPa (10-B3)
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PurificationAffinity purified
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applicationsImmunohistochemistry (IHC), Western blot (WB)
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Connected productsAS13 2640 | anti-ACT | actin, rabbit antibody AS10 681 | Anti-tubulin beta chain, rabbit antibodyAS10 680 | Anti-tubulin alpha chain, rabbit antibodyAS10 702 | Actin-11, mouse monoclonal antibodyAS16 3140 | ACT2,8,11 | Actin 1, 8, 11, mouse monoclonal antibodyAS16 3139 | ACT1,3,4,12 | Actin 1, 3, 4, 12, mouse monoclonal antibodyPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use1-2 ug/ml (WB)
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Molecular weight expected аpparent45 | 45 kDa
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Verified reactivityArabidopsis thaliana, Dictyostelium discoideum
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Possible reactivityActinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pratense, Ziziphus jujuba
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No reactivityno confirmed exceptions from predicted reactivity are currently known
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Supplementary informationContact Gentaur support center at [email protected]
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ReferencesKandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.
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Scientific contextActin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
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NotesThis antibody is a total IgG fraction purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin
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Protein numberQ96293
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TAIR numberAt1g49240
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Gene target
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Gene symbolSERPINA3, POTEM, FHL5, CISTR, ACOT7, CCL4, TRS-ACT1-1
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Short nameACT, Actin (monoclonal)
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TechniqueMonoclonals or monoclonal antibodies
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Alternative nameACT, Actin (monoclonal)
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Gene info
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Identity
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Gene
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Long gene nameserpin family A member 3
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Synonyms gene
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Synonyms gene name
- alpha-1-antichymotrypsin
- serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
- serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePOTE ankyrin domain family member M
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Synonyms gene name
- POTE ankyrin domain family, member M
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Synonyms
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Synonyms name
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Locus
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Discovery year2009-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- POTE ankyrin domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefour and a half LIM domains 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-26
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Entrez gene record
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RefSeq identity
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Classification
- LIM domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namechondrogenesis-associated transcript
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2014-01-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameacyl-CoA thioesterase 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Acyl-CoA thioesterases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 4
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Synonyms gene
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Synonyms gene name
- small inducible cytokine A4 (homologous to mouse Mip-1b)
- chemokine (C-C motif) ligand 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-07-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Ser (anticodon ACT) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA serine 32 (anticodon ACU) pseudogene
- transfer RNA-Ser (ACT) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
