ACP5/TRAP, human recombinant

  • Catalog number
    P1055-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP
  • Alternative_names
    Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP
  • Description
    An enzyme expressed in bone-resorbing osteoclasts and involved in osteopontin/bone sialoprotein dephosphorylation.
  • Recombinant
    Yes
  • Source
    Baculovirus
  • Purity by SDS PAGE
    > 95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    Measured by its abolity to hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
  • Biological activity
    Specific activity is > 5,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
  • Results
    > 5,000 units/mg
  • Unit Definition
    One unit is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
  • Molecular Weight
    35.1 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Liquid
  • Physical form description
    In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
  • Background Information
    ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
  • Amino acid sequence
    ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNHDHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVLVAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGGFAYVEISSKE MTVTYIEASG KSLFKTRLPR RARPHHHHHH
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    ACP5, TGFBRAP1, CD40LG, SCYL1
  • Short name
    ACP5/TRAP, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    acid phosphatase 5, tartrate resistant/TRAP, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    acid phosphatase 5, tartrate resistant, SPENCDI and TRAP, ACP5 and IDBG-29654 and ENSG00000102575 and 54, hydrolase activity, Plasma membranes, Acp5 and IDBG-143414 and ENSMUSG00000001348 and 11433, ACP5 and IDBG-643580 and ENSBTAG00000004826 and 517002
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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