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Target antigen
ABCA1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1(2219-2236aa NFAKDQSDDDHLKDLSLH), identical to the related rat and mouse sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the ABCA1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The ABCA1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
ABCA1(ATP-binding cassette, sub-family A(ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein(CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.
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Related articles
1. Langmann, T., Klucken, J., Reil, M., Liebisch, G., Luciani, M.-F., Chimini, G., Kaminski, W. E., Schmitz, G. Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages. Biochem. Biophys. Res. Commun. 257: 29-33, 1999. 2. Szakacs, G., Langmann, T., Ozvegy, C., Orso, E., Schmitz, G., Varadi, A., Sarkadi, B. Characterization of the ATPase cycle of human ABCA1: implications for its function as a regulator rather than an active transporter. Biochem. Biophys. Res. Commun. 288: 1258-1264, 2001.
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Gene Name
ABCA1
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Protein Name
ATP-binding cassette sub-family A member 1
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Gene Full Name
ATP-binding cassette, sub-family A(ABC1), member 1
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Synonyms
ABC 1 antibody|ABC Transporter 1 antibody|ABC-1 antibody|ABC1 antibody|ABCA 1 antibody|ABCA1 antibody|ABCA1_HUMAN antibody|ATP binding Cassette 1 antibody|ATP binding cassette sub family A ABC1 member 1 antibody|ATP binding cassette sub family A member 1 antibody|ATP binding cassette sub-family A member 1 antibody|ATP binding Cassette Transporter 1 antibody|ATP-binding Cassette 1 antibody|ATP-binding cassette sub-family A member 1 antibody|ATP-binding Cassette Transporter 1 antibody|CERP antibody|Cholesterol Efflux Regulatory Protein antibody|FLJ14958 antibody|HDLDT1 antibody|Membrane bound antibody|MGC164864 antibody|MGC165011 antibody|TD antibody|TGD antibody
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Uniprot ID
O95477
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
