AAV DJ 8 Helper Free Expression System

AAV DJ 8 Helper Free Expression System is available 8 times from Cellular biology labs labs

VPK-419-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Blasticidin)size: 1 test kit | 1613.52 USD

  • SDS or safty data sheet
    more (VPK-410 through VPK-430-SER6) GHS.pdf
  • Related techniques
    Viral Expression, Adeno-Associated Virus (AAV) Expression, AAV Helper Free Complete Expression Systems, AAV-DJ/8 Helper Free Complete Expression Systems
  • Use of the reagent
    The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
  • Cellular use
    Complete Expression System contains packaging plasmids, expression vector and GFP control vector
  • Protocol information
    pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
  • Additional lab advice
    Cloning capacity of expression vector = 2 kb
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,51
1. Gene info
  • Identity
  • Gene
  • Long gene name
    adeno-associated virus integration site 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1990-09-10
  • Entrez gene record
  • Pubmed identfication
Additional information

VPK-418-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (GFP)size: 1 test kit | 1613.52 USD

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VPK-417-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)size: 1 test kit | 1613.52 USD

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VPK-416-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Neo)size: 1 test kit | 1613.52 USD

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VPK-415-DJ-8 | AAV-DJ/8 Helper Free Bicistronic Expression System (Puro)size: 1 test kit | 1613.52 USD

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VPK-410-DJ-8 | AAV-DJ/8 Helper Free Expression Systemsize: 1 test kit | 1613.52 USD

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VPK-400-DJ-8 | AAV-DJ/8 Helper Free Packaging Systemsize: 1 test kit | 1264.55 USD

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VPK-411-DJ-8 | AAV-DJ/8 Helper Free Promoterless Expression Systemsize: 1 test kit | 1613.52 USD

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  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    -20ºC
  • Type of reagent
    Viral Expression
  • Reagent properties
    AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
  • Living modified organisme in this reagent or LMO
    Not applicable
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
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