7-Ketocholesterol Antibody: Biotin

Alternative Name

7-Ketocholesterol Antibody, Clone 7E1: Biotin
Other name

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b
Target

7-Ketocholesterol
Conjugate

Biotin
Category

Antibodies
Product Type

Monoclonal
Clone Number

7E1
Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Species

NA
Gene ID

NA
Swiss Prot

NA
Applications

WB , ICC/IF , ELISA
Host Species

Mouse
Species Reactivity

Species Independent
Storage Buffer

PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration

1 mg/ml
Specificity

Specific for 7-ketocholesterol modified proteins.
Storage Temperature

-20°C
Shipping Temperature

Blue Ice or 4°C
Properties

If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data