6x, Histidine, HRP conjugated
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Catalog number
AS15 2930
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Price
Please ask
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Size
100 µg
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Available ordering format
Lyophilized
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Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Clone AD1.1.10
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Purification
Protein A purified
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How to reconstitute
For reconstitution att 1 ml of sterile water to a final concentration of 0.1mg / mlAfter reconstitution let the product sit on ice for 20 minutes.
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Storage condition
Store at 2-8°C in the dark.Shelf life: 6 months from date of receipt. Prepare working dilution prior to use and then discard.
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Verified applications
Western blot (WB)
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Connected products
AS15 2936 | 6x Histidine, Unconjugated (1 mg) (mouse antibody)AS15 2935 | 6x Histidine, ALP Conjugated (mouse antibody)AS15 2934 | 6x Histidine, Biotin Conjugated (mouse antibody)AS15 2928 | 6x Histidine, conjugated to DyLight® 488 (mouse antibody)AS15 2929 | 6x Histidine, conjugated to DyLight® 550 (mouse antibody)AS15 2933 | 6x Histidine, conjugated to DyLight® 650 (mouse antibody)AS15 2932 | 6x Histidine, conjugated to Europium (mouse antibody)AS15 2931 | 6x Histidine, conjugated to SureLight® R-Phycoerythrin (mouse antibody)other tag antibodies
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Recommended dilutions for use
1: 1000 (WB)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in August 2015.
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Scientific context
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to HRP. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
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Notes
Antibody is provided in: 110 mM Sodium Phosphate (pH 7.4), 100 mM Sodium Chloride, 50 mM Sucrose, 0.1% BSA and 2 mM sodium azide
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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Gene target
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Short name
6x, Histidine, conjugated
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Technique
antibody Conjugates
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Label
HRP
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Alternative name
6x, Histidine, horseradish peroxidase coupled
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