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Available ordering format
Lyophilized
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Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Clone AD1.1.10
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Purification
Protein A purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
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Verified applications
Western Blot (WB)
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Connected products
AS15 2936 | 6x Histidine, Unconjugated (1 mg) (mouse antibody)AS15 2930 | 6x Histidine, HRP conjugated (mouse antibody)AS15 2935 | 6x Histidine, ALP Conjugated (mouse antibody)AS15 2928 | 6x Histidine, conjugated to DyLight® 488 (mouse antibody)AS15 2929 | 6x Histidine, conjugated to DyLight® 550 (mouse antibody)AS15 2933 | 6x Histidine, conjugated to DyLight® 650 (mouse antibody)AS15 2932 | 6x Histidine, conjugated to Europium (mouse antibody)AS15 2931 | 6x Histidine, conjugated to SureLight® R-Phycoerythrin (mouse antibody)other tag antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in August 2015
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Scientific context
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to Biotin. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
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Notes
Antibody is provided in: 100 mM Sodium Phosphate (pH 7.4), 100 mM Sodium Chloride, 50 mM Sucrose, 0.1% BSA and 2 mM Sodium Azide
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated