6-ROXtra fluorescence reference solution *25 uM for PCR reactions*
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Catalog number
398
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Price
Please ask
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Size
5 ml
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Description
6-ROXtra fluorescence reference solution *25 uM for PCR reactions* is a purple solution which is used as a reference dye for performing PCR detections.
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Shipping
Room Temperature
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Storage
Freezer
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Shelflife
12 months
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Molecular Weight KD
844.80
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Appearance
Purple solution
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Solubility
Soluble In: Water
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Excitation Wavelength
575 nm
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Emission Wavelength
602 nm
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Personal precautions
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order 6-ROXtra fluorescence reference solution *25 uM for PCR reactions*, please use the Cat. Nr.398 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for 6-ROXtra fluorescence reference solution *25 uM for PCR reactions*
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Test
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Additional description
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
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Gene target
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Short name
6-ROXtra reference *25 uM for PCR *
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Technique
PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative name
6-ROXtra fluorescence reference solution *25 uM to measure PCR test kit reactions*
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Alternative technique
dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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