5-Fluorescein isothiocyanate isomer I (FITC)

CAS number

[3326-32-7]
Availability

Please contact Gentaur/Genprice to check the latest availability
Ordering

For ordering the 5-Fluorescein isothiocyanate isomer I (FITC) please use the Catalogue ID#43825432 in your purchase order among with the desired quantity, your shipping and billing addresses, as well applicable VAT number. Please contact Gentaur for bulk or larger quantity, a discount may apply.
Technical files

Please contact our technical support to request the latest datasheet, certificate of analysis or the material safety datasheet
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Properties

This Molekula Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Molekula FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data