Coombs, Clone: Z-1, Rabbit polyclonal; ELISA/WB/IHC/flow/IP

• Catalog number
  YVG146-A
• Price
  Please ask
• Size
  0.1mg

• Alternative name 1
  Coombs, Clone: Z-1, Rabbit polyclonal; ELISA/WB/IHC/flow/IP
• Alternative name 2
  Coombs, Clone: Z-1, Rabbit polyclonal; ELISA/WB/IHC/flow/IP
• Alternative name 3
  Coombs, Clone: Z-1, Rabbit polyclonal; ELISA/WB/IHC/flow/IP
• Alternative name 4
  anti-Coombs Clone: Z-1 polyclonal //C/flow
• Alternative name 5
  Coombs Clone: Z-1 polyclonal //C/flow
• Clonality
  Monoclonal Antibody
• Description
  This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
• Storage and handling
  Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
• Advisory
  To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  Coombs   Z-1   IHC  
• Gene symbol
  HLA-Z, PSMB7, PLA2G4F, ZNF276, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1
• Short name
  Coombs, Clone: Z-1, Rabbit polyclonal; ELISA/ /IHC/ /IP
• Technique
  Polyclonal, Rabbit, ELISA, Flow, ihc, IP, WB, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Immunoprecipitation, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Western Blot on a membrane
• Host
  Rabbit, antigen assay ELISA kit 1 Kit (96 Wells), Rabbits
• Alternative name
  Coombs, clonality: Z-1, production species: rabbit polyclonal; Enzyme-linked immunosorbent assay/Western Blot/Immunohistochemistry/flow/IP
• Alternative technique
  polyclonals, rabbit-anti, elisas

Gene info

• Identity
  HGNC:19394
• Gene
  HLA-Z
• Long gene name
  major histocompatibility complex, class I, Z (pseudogene)
• Synonyms
  • HLA-Z1
• Locus
  6p21.32
• Discovery year
  2002-10-23
• Entrez gene record
  267017
• Pubmed identfication
  • 8568858
• RefSeq identity
  • NG_009944
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031189
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:9544
• Gene
  PSMB7
• Long gene name
  proteasome 20S subunit beta 7
• Synonyms gene name
  • proteasome (prosome, macropain) subunit, beta type, 7
  • proteasome subunit beta 7
• Synonyms
  • Z
• Synonyms name
  • proteasome subunit β2
• GenBank acession
  • AJ420455
• Locus
  9q33.3
• Discovery year
  1995-11-22
• Entrez gene record
  5695
• Pubmed identfication
  • 8811196
• RefSeq identity
  • NM_002799
• Classification
  • Proteasome
• VEGA ID
  OTTHUMG00000021042

Gene info

• Identity
  HGNC:27396
• Gene
  PLA2G4F
• Long gene name
  phospholipase A2 group IVF
• Synonyms gene name
  • phospholipase A2, group IVF
• Synonyms
  • PLA2G4F/Z
• Locus
  15q15.1
• Discovery year
  2005-11-04
• Entrez gene record
  255189
• Pubmed identfication
  • 14702039
  • 15866882
• RefSeq identity
  • NM_213600
• Classification
  • C2 domain containing phospholipases
  • Phospholipases
• VEGA ID
  OTTHUMG00000172782

Gene info

• Identity
  HGNC:23330
• Gene
  ZNF276
• Long gene name
  zinc finger protein 276
• Synonyms gene
  • ZFP276
• Synonyms gene name
  • zinc finger protein 276 homolog (mouse)
• Synonyms
  • MGC45417
  • ZNF477
  • CENPZ
  • CENP-Z
  • ZADT
• Synonyms name
  • centromere protein Z
  • zinc finger, AD-type
• GenBank acession
  • AK026482
• Locus
  16q24.3
• Discovery year
  2003-11-07
• Entrez gene record
  92822
• Pubmed identfication
  • 10936049
  • 20813266
• RefSeq identity
  • NM_152287
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000138050

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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