12 Hydroxyeicosatetraenoic Acid 12 HETE ELISA Kit 96 well plate

12 Hydroxyeicosatetraenoic Acid 12 HETE ELISA Kit 96 well plate is available 3 times from Bioma labs

EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well platesize: 10x96T | 8733.41 USD

  • Catalog number
    EKU08719
  • Supplier
  • Price
    8733.41 USD
  • Size
    10x96T
1. Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note:An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
Additional information

EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well platesize: 5x96T | 4829.42 USD

Ajax processing

EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well platesize: 96T | 1142.97 USD

Ajax processing
  • Verified reactivity
    Multispecies (General)
  • Detection limits
    12.35-1000 ng/mL
  • Sensitivity limit
    4,64 ng/mL
  • Samples to be analyzed
    Serum, plasma and other biological fluids
  • Assay duration
    2 hours
  • Verified applications
    ELISA
  • Assay class
    Competitive
  • ELISA detection
    Colorimetric
  • Protein number
    Please refer to Uniprot
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    Please refer to GenBank
  • Gene name
    12-Hydroxyeicosatetraenoic Acid
  • Alternate gene name
    12HETE
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA's specificity
    This assay has high sensitivity and excellent specificity for detection of 12-Hydroxyeicosatetraenoic Acid (12-HETE). No significant cross-reactivity or interference between 12-Hydroxyeicosatetraenoic Acid (12-HETE) and analogues was observed.
  • ELISA's cross-reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) and unlabeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) (Standards or samples) with the pre-coated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample.
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 12-Hydroxyeicosatetraenoic Acid (12-HETE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 12-Hydroxyeicosatetraenoic Acid (12-HETE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA's stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
  • Warnings
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Description
    A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
  • Gene target
  • Short name
    12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96
  • Technique
    ELISA kit, ELISA
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    12-Hydroxyeicosatetraenoic Acid (12-HETE) Enzyme-linked immunosorbent assay reagent, 96 well plate
  • Alternative technique
    elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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