10x PCR Buffer with MgCl2

  • Catalog number
    ABP-PP-ERBFM02
  • Price
    Please ask
  • Size
    2x1ml
  • You will recive this
    Ice Packs
  • Product Family
    PCR/Cloning Reagents
  • Name of the reagent
    PCR Reagents
  • Description
    Buffering solutions are useful to keep the pH range sable when using this reagent of allele.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
    10x   with   MgCl2  
  • Short name
    10x PCR with MgCl2
  • Technique
    PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Alternative name
    10x PCR test kit solution including MgCl2
  • Alternative technique
    dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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